TEL - AMLI Translocations With TEL and CDKN 2 Inactivation in Acute Lymphoblastic Leukemia Cell Lines
نویسندگان
چکیده
The t(12;21)(p13;q22) results in the fusion of the TEL gene located on chromosome 12 with the AMLI gene located on the derivative chromosome 21. Because this translocation is difficult to detect using standard cytogenetic techniques, 27 previously karyotyped Blineage acute lymphoblastic leukemia (ALL) cell lines were evaluated for the presence of the EL-AMLI fusion using the reverse transcriptase-polymerase chain reaction (RT-ER), fluorescence in situ hybridization (FISH), and cDNA sequencing. Six cell lines expressed the TELAML1 chimeric transcript by RT-PCR and the t(12;21) was confirmed by FISH analysis with probes for TEL, AMLI, and chromosome 12. While only one of the 6 cell lines with the t(12;21) lost the der(l2)t(l2;21)-encoded AMtl-TEL fusion transcript, 4 cell lines lacked expression of the non-
منابع مشابه
TEL-AML1 translocations with TEL and CDKN2 inactivation in acute lymphoblastic leukemia cell lines.
The t(12;21) (p 13; q22) results in the fusion of the TEL gene located on chromosome 12 with the AML1 gene located on the derivative chromosome 21. Because this translocation is difficult to detect using standard cytogenetic techniques, 27 previously karyotyped B-lineage acute lymphoblastic leukemia (ALL) cell lines were evaluated for the presence of the TEL-AML1 fusion using the reverse transc...
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